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Research Article

Materials and Methods Top

Materials. The following chemicals were purchased from Sigma Chemical Co. (St. Louis, MO, USA): sodium arsenite (As³⁺), sodium arsenate (As⁵⁺), dimethylarsinic acid (DMA), acetylcholine, phenylephrine, sodium nitroprusside (SNP), 8-Br-cGMP, and Dowex AG50W-X8 (100-200 mesh). Monomethylarsonic acid (MMA) was obtained from Chem Service (West Chester, PA, USA), and a cGMP radioimmunoassay kit was obtained from Amersham (Buckinghamshire, UK). Human aortic endothelial cells (HAEC) and the endothelial cell growth media (EGM) kit were purchased from Clonetics Corporation (Walkersville, MD, USA). Minimum essential media (MEM) was supplied by Life Technologies (Rockville, MD, USA), and all other reagents used were of the highest purity available.

Animals. We used male Sprague-Dawley rats (Dae Han BioLink, Chungbuk, Korea) weighing 300-400 g in all experiments. Before the experiments, the animals were acclimated for 1 week in a laboratory animal facility maintained at constant temperature and humidity with a 12-hr light/dark cycle. Food and water were provided ad libitum.

Preparation of blood vessels in organ bath. Rats were killed by decapitation and then exsanguinated. We carefully isolated the thoracic aorta and cut it into ring segments. Aortic rings without endothelium were prepared by gently rubbing the intimal surface of the aortic rings with a wooden stick. The aortic rings were treated with As³⁺ or the vehicle (saline) in MEM with 100 U/mL penicillin and 100 µg/mL streptomycin at 37°C in a 95% air/5% CO₂ incubator for 14 hr. The rings were then mounted in four-channel organ baths filled with Krebs-Ringer solution (pH 7.4): 115.5 mM NaCl, 4.6 mM KCl, 1.2 mM KH₂PO₄, 1.2 mM MgSO₄, 2.5 mM CaCl₂, 25.0 mM NaHCO₃, and 11.1 mM glucose. The organ baths were continuously gassed with 95% O₂/5% CO₂ and maintained at 37°C. The rings were stretched gradually to an optimal resting tension of 2 g and equilibrated for 30 min. We measured the change in tension isometrically with Grass FT03 force transducers (Grass Instrument Co., Quincy, MA, USA) and recorded the change using the AcqKnowledge III computer program (BIOPAC Systems Inc., Goleta, CA, USA).

To investigate the effect of As³⁺ on endothelium-dependent relaxation, acetylcholine was used as an agonist. The aortic rings were precontracted submaximally by adding phenylephrine, and acetylcholine was then cumulatively added to the organ bath to obtain concentration-relaxation curves. To examine the effects of As³⁺ on the NO donor-induced relaxation and on direct cGMP-dependent relaxation, sodium nitroprusside was used as the NO donor and 8-bromoguanosine 3´,5´-cyclic monophosphate (8-Br-cGMP) was used as the cGMP analog. Relaxation was expressed as the percent decrease in contractile tone elicited by phenylephrine.

Determination of eNOS activity. We determined eNOS activity by measuring the conversion of [³H]-l-arginine to [³H]-l-citrulline. These amino acids were separated by anion exchange chromatography using a modification of the method of Bredt and Snyder (1990). We maintained HAEC (six to eight passages) in the EGM kit at 37°C in a 95% air/5% CO₂ incubator. Before the experiments, 4 × 10⁴ cells were seeded into 12-well plates and grown for 48 hr. After the HAEC were treated with various concentrations of As³⁺ for 14 hr, they were incubated for 20 min in HEPES buffer (pH 7.4): 140 mM NaCl, 2.7 mM KCl, 1.0 mM MgCl₂, 5.0 mM glucose, 25 mM HEPES, and 3 µCi/mL [³H]-l-arginine. The cells were washed twice with ice-cold Ca²⁺-free buffer containing 5 mM EDTA, and then 0.3 M HClO₄ was added. The tissue extract was then neutralized with 3 M K₂CO₃ and centrifuged at 12,000 × g for 2 min; the supernatants were applied to columns containing Dowex AG50W-X8 (Na⁺ form), and the eluted [³H]-l-citrulline was measured by scintillation counting. We determined protein content by the method of Lowry et al. (1951).

Measurement of superoxide anion generation. We determined generation of superoxide anions by lucigenin-induced chemiluminescence. Aortic rings were placed in a Krebs-Ringer solution continuously gassed with 95% O₂/5% CO₂ and allowed to equilibrate for 30 min at 37°C. A mixture of 2 mL Krebs-Ringer solution with 0.25 mM lucigenin was prepared in a scintillation tube and mixed with various concentrations of As³⁺. Aortic rings were then added to each tube, and chemiluminescence was measured for 60 min using a luminometer (Berthold, Germany).

Measurement of cGMP levels. We treated rat aortic rings with As³⁺ or saline for 14 hr as described previously (Lee et al. 2001). For the experiments in which we examined cGMP levels stimulated by an agonist, 10^-7 M acetylcholine was added to the organ bath for 1 min. The reactions were immediately stopped with liquid nitrogen, and the tissue was homogenized in 1 mL ice-cold 6% trichloroacetic acid. The homogenate was centrifuged at 13,600 × g for 15 min at 4°C. The supernatant was extracted with water-saturated ether. We assayed extracts for cGMP levels by radioimmunoassay (RIA) using a [¹²⁵I]-cGMP RIA kit (Amersham) according to the procedure described by the manufacturer. We determined protein content from the pellet according to the method of Lowry et al. (1951).

Measurement of blood pressure change induced by acetylcholine. Rats were anesthetized with phenobarbital (50 mg/kg, intraperitoneal). A catheter of polyethylene PE-50 tubing (Clay Adams, Sparks, MD, USA) filled with heparinized saline (100 U/mL) was placed in the carotid artery for the measurement of blood pressure, and a catheter of polyethylene PE-10 fused to PE-50 tubing was placed in the jugular vein for the administration of drugs. Catheters were tunneled subcutaneously and exteriorized at the back of the neck. Wounds were sutured and cleaned with alcohol. We began experiments after a 1-day recovery period. On the day of the experiment, the arterial catheter was connected to a pressure transducer (BIOPAC Systems Inc.), and blood pressure was measured using the AcqKnowledge III computer program. Blood pressure was allowed to stabilize for a minimum of 30 min before treatment began. To determine the effects of As³⁺ on blood pressure reduction induced by acetylcholine, we administered As³⁺ solution (1 mg/kg) by an intravenous bolus injection into the jugular vein. In the controls, equivalent amounts of saline were injected. After 2 hr, the rats were infused with 10 µg/kg/min acetylcholine for 2 min via the jugular vein, and the change in blood pressure in response to acetylcholine was monitored simultaneously. Infusions were performed with a Harvard syringe pump (Southnatick, MA, USA) at a rate of 0.1 mL/min.

Statistical analysis. We calculated the means and standard errors of means for all treatment groups. The data were subjected to one-way analysis of variance followed by Duncan's multiple range test to determine which means were significantly different from the control. In all cases, a p value of < 0.05 was used to determine significance.

Results Top

To determine whether arsenic affects relaxation of blood vessels, we treated intact aortic rings with various concentrations of As³⁺ for 14 hr. Phenylephrine was applied to precontract the rings, followed by cumulative addition of acetylcholine to obtain concentration-relaxation curves (Figure 1A). As³⁺ suppressed the relaxation induced by acetylcholine in a concentration-dependent manner. We also investigated the effects of the pentavalent inorganic species (As⁵⁺) and two major metabolites, MMA and DMA, on the inhibition of acetylcholine-induced vasorelaxation (Figure 1B). However, As³⁺, MMA, and DMA failed to inhibit the acetylcholine-induced relaxation.

Figure 1.

Inhibitory effect of As³⁺ on relaxation of aortic rings induced by acetylcholine. After intact aortic rings were treated with (A) various concentrations of As³⁺ and (B) other species of arsenic (100 µM each) for 14 hr, 1 µM phenylephrine was added to precontract the aortic rings and then increasing concentrations of acetylcholine were added in a cumulative manner. Concentration-response curves to acetylcholine were determined with relaxation expressed as the percent decrease in contractile tone elicited by phenylephrine. The values of curves are means ± SEM of four to five independent experiments.

Acetylcholine stimulates eNOS to produce NO in endothelium, which results in the relaxation of smooth muscle (Wanstall et al. 2001). To examine whether As³⁺ inhibits NO synthesis by endothelial cells, we investigated the effect of As³⁺ on eNOS activity in cultured HAEC. As³⁺ reduced eNOS activity in a concentration-dependent manner (Figure 2A), suggesting that As³⁺ can suppress vascular NO production in endothelial cells. Others have reported that As³⁺ can generate superoxide anions in cultured endothelial cells and smooth muscle cells (Lynn et al. 2000; Smith et al. 2001). Superoxide anions interact with NO to form peroxynitrite, resulting in suppression of vascular relaxation by blocking the NO pathway (Pryor and Squadrito 1995). To determine if superoxide anions generated by As³⁺ may play a role in the suppression of vascular relaxation, we treated aortic rings with intact endothelium with As³⁺, and superoxide production was evaluated by measuring lucigenin-induced chemiluminescence. Increased chemiluminescence was not observed at any concentration of As³⁺ tested (Figure 2B

Figure 2.

Effects of As³⁺ on eNOS activity in human aortic endothelial cells and superoxide production in aortic rings. (A) After As³⁺ was added to HAEC for 14 hr, the activity of eNOS was determined by the formation of l-citrulline from l-arginine, as described in "Materials and Methods." (B) Several concentrations of As³⁺ were added to aortic rings and superoxide anions was measured by lucigenin-induced chemiluminescence. Menadione (MEN; 10 µM) was used as positive control. Values are means ± SEM of (A) five to six and (B) three independent experiments.

NO released from endothelium elicits vascular relaxation by increasing the levels of cGMP in smooth muscle (Robertson et al. 1993). To determine the effects of As³⁺ on the NO-dependent pathway, we measured the accumulation of cGMP in aortic rings. The basal cGMP levels in the aortic rings were significantly decreased by As³⁺ treatment in a concentration-dependent manner (Figure 3A). After stimulation with 10^-7 M acetylcholine, As³⁺ again significantly reduced cGMP levels, even though the cGMP levels were 30 times greater due to the acetylcholine stimulation (Figure 3B). However, unlike the results for acetylcholine-induced relaxation (Figure 1A), no difference in cGMP level was found between the 25- and 50-µM As³⁺ treatments, suggesting that arsenic may interfere with some relaxation mechanism in addition to inhibiting endothelium-dependent NO production.

Figure 3.

Effects of As³⁺ on cGMP levels of aortic rings. After aortic rings were treated with As³⁺ for 14 hr, (A) cGMP levels in basal state and (B) cGMP levels accumulated by 10^-7 M acetylcholine were determined by RIA method as described in "Materials and Methods." Values are means ± SEM of five to seven independent experiments.

Therefore, using aortic rings without endothelium, we examined the effect of As³⁺ on relaxation induced by SNP, a direct NO-releasing agent. Treatment with As³⁺ inhibited vasorelaxation induced by SNP to a lesser extent (Figure 4A) than the vasorelaxation induced by acetylcholine. As³⁺ at a concentration of 25 µM did not suppress SNP-induced relaxation, but 50 µM As³⁺ showed significant inhibition. Consistent with this finding, when we investigated the effect of As³⁺ on relaxation induced by the cGMP analog, 8-Br-cGMP in aortic rings without endothelium, only 50 µM As³⁺ resulted in significant reduction of cGMP analog-induced relaxation (Figure 4B). These results suggested that 50 µM As³⁺ could interfere with the cGMP-dependent relaxation machinery of smooth muscle in blood vessels.

Figure 4.

Effects of As³⁺ on relaxation induced by sodium nitroprusside and 8-Br-GMP in aortic rings without endothelium. After aortic rings without endothelium were treated with As³⁺ for 14 hr, 1 µM phenylephrine was added to precontract the aortic rings and then relaxation was elicited by (A) cumulative addition of SNP, NO donor and (B) 30 µM 8-Br-cGMP, a cGMP analog. Relaxation is expressed as percentage of decrease in contractile tone elicited by phenylephrine. Values are means ± SEM of four independent experiments.

To verify the effects of As³⁺ on blood vessels in vivo, we monitored the change in blood pressure after intravenous infusion of acetylcholine into conscious rats (Figure 5). An intravenous bolus of As³⁺ had no effect on basal blood pressure (Figure 5A). When rats were infused with 10 µg/kg/min acetylcholine 2 hr after As³⁺ treatment (Figure 5C), the reduction of blood pressure induced by acetylcholine was significantly suppressed compared to the control group (10.8 ± 3.5 vs. 31.3 ± 4.7 mmHg) (Figure 5B). These results suggest that As³⁺ caused suppression of vasorelaxation induced by acetylcholine in vivo. This confirms the previous in vitro results shown in Figure 1A.

Figure 5.

Effect of intravenously administered As³⁺ on decrease in blood pressure by acetylcholine infusion in rats. (A) Blood pressure changes by As³⁺ bolus and saline infusion were monitored. Decrease in blood pressure by acetylcholine infusion (10 µg/kg/min) was measured via carotid artery 2 hr after an intravenous bolus of (B) saline or (C) As³⁺ [1 mg/kg]. Infusions were performed by Harvard syringe pump at a rate of 0.1 mL/min for 2 min (indicated by line). Data are representative tracings of five independent experiments.

Discussion Top

This is the first study to evaluate the effects of As³⁺ on vasomotor function in vitro and in vivo. We have shown that As³⁺ inhibits the vascular relaxation induced by acetylcholine in a concentration-dependent manner and that possible mechanisms are the inhibition of NO production in endothelial cells and the suppression of cGMP-dependent relaxation mechanisms in smooth muscles. These in vitro results were consistent with in vivo results (Figure 5) in which As³⁺ suppressed the reduction of blood pressure by acetylcholine in conscious rats. Our findings correlate well with a previous study reporting that serum concentrations of NO metabolites were decreased in a population exposed to arsenic in drinking water (Pi et al. 2000). In addition, this As³⁺-induced suppression in vitro was still observed after the removal of residual As³⁺ by changing the incubation medium, suggesting that the As³⁺-induced effect was irreversible and that damaged blood vessels might not recover even after As³⁺ has disappeared from the bloodstream.

Several epidemiologic studies have reported that arsenic-contaminated drinking water causes various types of cardiovascular disease such as blackfoot disease, atherosclerosis, cerebrovascular disorders, and hypertension (Chen et al. 1988; Chiou et al. 1997; Rahman et al. 1999; Tseng 1977; Wang et al. 2002). Recently, the effect of arsenic on platelets has been suggested as a key mechanism in the development of these cardiovascular diseases (Lee et al. 2002). Blood vessels, however, are another tissue important in the development of cardiovascular diseases. Dysfunction in blood vessels disrupts the balance in vasomotor tone between relaxation and contraction, with vasoconstriction predominating and ultimately leading to possible increased risk for development of vascular diseases such as hypertension and atherosclerosis (Lefer et al. 1991; Luscher et al. 1993; Rubanyi 1993; Vanhoutte 1997). Because our data showed that As³⁺ could suppress endothelium-dependent vasorelaxation with subsequent changes in vasomotor tone in blood vessels, we propose arsenic-induced dysfunction in blood vessels as an alternative mechanism for arsenic-associated cardiovascular disease observed in human populations.

Treatment with As³⁺ did not result in concentration-dependent inhibition of vasorelaxation induced by SNP (Figure 4A) compared to the results obtained by acetylcholine (Figure 1A). As³⁺ at a concentration of 25 µM did not inhibit SNP-induced relaxation but did inhibit acetylcholine-induced relaxation, suggesting that 25 µM As³⁺ may interfere with NO production in endothelial tissue. This conclusion is supported by our finding that As³⁺ can significantly inhibit eNOS activity in cultured human aortic endothelial cells (Figure 2A). In addition to interfering with NO generation in aortic endothelium, higher concentrations of As³⁺ may also disrupt the relaxation mechanisms in smooth muscles. Several lines of evidence support this view. First, only 50 µM concentrations of As³⁺ showed an inhibitory effect on SNP-induced relaxation in aortic rings without endothelium (Figure 4A). Second, unlike acetylcholine-induced vasorelaxation (Figure 1A), levels of acetylcholine-stimulated cGMP in aortic rings showed no difference between treatments with 25 or 50 µM As³⁺Figure 3B). Third, treatment with 50 µM As³⁺ resulted in significant reduction of cGMP analog-induced vasorelaxation in aortic rings without endothelium (Figure 4B). These results suggest that impairment of NO generation in endothelium and impairment of cGMP-dependent relaxation mechanisms in smooth muscles depended on the concentrations of As³⁺ that blood vessels were exposed to.

As³⁺ is reported to stimulate the formation of reactive oxygen species (ROS) in vascular endothelial and smooth muscle cells, mainly via NADH/NADPH oxidase (Lynn et al. 2000; Smith et al. 2001); thus, the generation of superoxide could elicit the reduced relaxation due to elimination of NO. Therefore, we considered the possibility that superoxide production is involved in suppression of vasorelaxation by As³⁺. However, we detected no significant increase in ROS generation in intact aortic rings when measuring superoxide anions with the chemiluminescent probe lucigenin (Figure 2B). These contradictory results might be explained by the differences in experimental systems (we used aortic rings in an organ-bath system and the previous studies used a cell culture system), but the exact reason for this discrepancy is currently unknown. In any case, our results suggest that superoxide generation does not play an important role in the suppression of vasorelaxation by As³⁺.

Our study revealed that higher concentrations of As³⁺ inhibited cGMP-dependent relaxation of smooth muscle in aortic rings isolated from rats. Arsenic is well known to induce heat-shock proteins (Del Razo et al. 2001), a large family of proteins whose expression is usually induced by cellular stress. Knoepp et al. (2000) reported that smooth muscle relaxation induced by SNP and forskolin, a adenylate cyclase activator, was inhibited by cellular stresses such as heat shock and As³⁺ via inhibition of the phosphorylation of heat shock protein 20, which is a regulatory component of the actin-associated cytoskeleton. However, those effects were observed in bovine carotid artery, whereas our aortic rings were isolated from rats. Furthermore, 0.5 mM As³⁺ was used in those experiments, which was 10 times higher than the concentrations used in our experiment. In fact, treatment with 0.5 mM As³⁺ resulted in complete impairment of phenylephrine-induced precontraction in rat artery (data not shown), and thus the relaxation experiment by acetylcholine was not feasible. Therefore, it appears inappropriate to apply the explanation of Knoepp's group to our observation that 50 µM As³⁺ could suppress the relaxation machinery in smooth muscles. The exact mechanism remains to be identified.

Previous study reported that the normal concentration of arsenic in human plasma is 2.4 ± 1.9 µg/L and that this level may be increased up to 38 µg/L with chronic arsenic exposure (Heydorn 1970). Our in vivo arsenic experiment showed significant suppression of acetylcholine-induced vasorelaxation after only a 2-hr exposure (Figure 5), at which time arsenic reached its plasma level of less than 100 µg/L (data not shown). This experimental arsenic exposure was therefore not more than a factor of 3 greater than arsenic levels to which human blood vessels are exposed after chronic intake of arsenic-contaminated drinking water. Considering the facts that humans are more sensitive to arsenic toxicity than are several other species (Chan and Huff 1997), the concentration of arsenic used in the current investigation could be well within the range to induce cardiovascular disease when humans drink arsenic-contaminated water for weeks or even years.

From this study, we determined that As³⁺ caused altered vascular tone by decreasing vasorelaxation. The inhibitory effects may be due to suppression of NO production mediated by eNOS inhibition in endothelial cells and interference of cGMP-dependent relaxation machinery in smooth muscles (Figure 6). In our in vivo study, As³⁺ treatment of rats blocked the acetylcholine-induced hypotensive effect. These results confirm our in vitro observations and suggest that reduced vasorelaxation may be a contributing factor in development of cardiovascular diseases in populations exposed to arsenic.

Figure 6.

Proposed mechanism for inhibitory effect of As³⁺ on vasorelaxation. Abbreviations: Ach, acetylcholine; GC, guanylate cyclase; GTP, guanosine triphosphate. As³⁺ causes altered vasomotor tone by decreasing vasorelaxation induced by Ach. This could be due to suppression of NO production mediated by eNOS inhibition in endothelial cells and interference of cGMP-dependent relaxation machinery in smooth muscles.

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